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Elsevier Science Publishing Co Inc Transketolases : Volume 722

Transketolase is an ubiquitous enzyme which has not yet finished revealing all its secrets.This Methods in Enzymology: Transketolases volume is devoted to the description of cutting-edge methods to its study in biochemistry.Several topics are addressed by world experts in their field. The first part of this volume deals with transketolase at the cellular level.The latest scientific progress on human transketolase TKL1 in relation to certain diseases and to its inhibition are discussed including the involvement of this enzyme in new metabolic pathways or as a diagnostic biomarker. The second part focuses on transketolase at the molecular level.The structural and mechanistic aspects linked to this enzyme are addressed using several approaches including molecular modelling and crystallography, for example.Finally, the experimental methodological approaches relating to the use of transketolase as a tool of choice in biocatalysis is presented.

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